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Centre for AIDS and Related Diseases

Mailing address :

Centre for AIDS & Related Diseases (CA&RD), National Centre for Disease Control, 22-Sham Nath Marg
Delhi-110054; Tele/ Fax: 91-11-23934517

     
Name, Designation & Contact Details of the Head of the Division :

Dr. Shashi Khare, MBBS, MD (Microbiology)
Additional Director
Tel No. (O): 91-11-23934517
e-mail : shashi.khare@hotmail.com
Fax : 91-11-23934517

     
:

 

 
Broad outline of activities of the Centre:
 
1. HIV/ AIDS related activities
  • Serological testing and confirmation of HIV infection for referred samples.
  • Dried Blood Spot testing for HIV Sentinel Surveillance
  • Blinded rechecking of diagnostic and sentinel surveillance HIV testing performed by designated laboratories in Delhi, Haryana, Rajasthan and J&K
  • Conduction of External Quality Assessment Scheme (EQAS) for HIV serology  for State Reference Laboratories (SRLs) and their Integrated Counseling & Testing Centres at Delhi, Haryana, Rajasthan and J&K
  • Preparation and characterization of panel for HIV/HBV/HCV test kits evaluation
  • Evaluation HIV/HBV/HCV test kits referred by NACO
  • Absolute CD4 T-Lymphocyte cell count in HIV-positive patients by flow cytometry
  • Diagnosis of common opportunistic infections in HIV-positive patients i.e. Cryptosporidium spp. Microsporidium spp. and P. jeroveci in stool and sputum respectively
  • Counseling and HIV testing of direct walk-in clients
  • Serological diagnosis of syphilis
  • HIV/HBV/HCV test kits storage facility to DSACS
  • Organization of training programmes for Microbiologists and Technical Staff of SRLs on serological testing for HIV, absolute CD4 T-lymphocyte count by flow cytometry and development of Quality Management System in HIV laboratories
  • Support to National Health Programmes e.g. NACO
  • Participation in National and International EQAS for:
    • HIV, HBV & HCV Serology
    • Syphilis Serology
    • Absolute CD4 T-Lymphocyte Count
 
2. Other activities
  • Centralized sample collection for different Divisions of NCDC
  • Support to courses run at NCDC i.e. MPH & FETP
 
Units within the Division

Sl. No

Unit

 

1

HIV Serology Laboratory& Quality Control Laboratory

2

National Reference Laboratory

3

Immunology Laboratory

 

4

STI and Opportunistic Infections Laboratory

5

Molecular Virology Laboratory

6

Integrated Counseling and Testing Centre (ICTC)

 

7

Central Sample Collection Unit

8

Test Kits Distribution Unit

 

Organogram of the Centre for AIDS & Related Diseases
 
Units 1 and 2 : HIV Serology and Quality Control Laboratory
  • Confirmation of HIV infection for samples referred by linked Non-SRL Laboratories 
  • Dried Blood Spot testing for HIV Sentinel Surveillance
    • Designated as one of the laboratory for testing of dried blood spot (DBS) samples under HIV Sentinel Surveillance programme of NACO
    • Twenty eight high risk group (HRG) sentinel sites spread across four states namely Jammu & Kashmir, Haryana, Rajasthan & Delhi have been linked to this centre
  • Blinded rechecking of diagnostic HIV testing performed by non-SRL laboratories
  • Blinded rechecking of sentinel surveillance HIV testing performed by designated laboratories in Delhi, Haryana, Rajasthan and J&K
 
Unit 3 : National Reference Laboratory
  • HIV/ HBV/ HCV Diagnostic Kits evaluation
    • A consortium of National Reference Laboratories (NRLs) comprising of NCDC, Delhi; NARI, Pune; NICED, Kolkata; NIMHANS, Bangalore and NIB, NOIDA has been formed
    • As part of the consortium activity, the center evaluates diagnostic kits (HIV/HBV/HCV), which are referred by NACO or by the procurement agency hired by NACO
    • Diagnostic kits submitted for evaluation is tested with a panel comprising of 500 well characterized members (100 positive and 400 negative samples)
    • Performance of the kit is evaluated by calculating various test indices
      • Sensitivity
      • Specificity
      • Delta values
  • Characterization of panel for kit evaluation
    • Regular collection of blood/ plasma  bags from various blood banks of Delhi
    • Characterized for developing panel for HIV/HBV/HCV kit evaluation
  • Conduction of External Quality Assessment Scheme (EQAS) for State Reference Laboratories and their linked Integrated Counseling & Testing Centers
    • 13 State Reference Laboratories (SRLs) in the four states viz. Delhi, Haryana, Rajasthan, Jammu & Kashmir and their associated ICTCs are linked with the National Reference Laboratory (NRL) at NCDC
    • EQAS programme is conducted twice a year for all the 13 SRLs and their associated ICTCs
    • A panel comprising of 08 member for each  of the SRLs and a bulk panel comprising of 04 members for ICTCs is sent after getting validated from NARI, Pune
    • Report of testing conducted on panel by SRLs is compiled and feedback is given to the respective SRLs with a copy to NACO & NARI, Pune
  • Blinded rechecking of diagnostic HIV testing performed by linked State Reference Laboratories 
  • Confirmation of HIV infection for samples referred by linked State Reference Laboratories 
  • Testing of blood-derived pharmaceutical products
    • Blood-derived pharmaceutical products referred by Drug Controller General (India) for testing for infectious markers i.e. HIV, HBV & HCV prior to batch release
    • Tested for various infectious markers (HIV, HBV & HCV)
  • Participation in EQAS for HIV serology conducted by the National AIDS Research Institute (NARI), Pune.
 
Unit 4 : Immunology Laboratory
  • Absolute CD4-T Lymphocyte count in HIV positive patients
    • Laboratory is linked with the Anti-retroviral (ART) centre of Deen Dayal Upadhyay (DDU) Hospital, Delhi and 8 PPTCT centers of Delhi
    • Absolute CD4-T Lymphocyte count is performed on samples received from these sites twice in a week i.e. on Tuesdays and Thursdays by flowcytometry using FACS Count machine
  • Participation in External Quality Assessment Programme (EQAS) for CD4/CD3 T-Lymphocyte count conducted by QASI, Canada, in association with National AIDS Research Institute (NARI), Pune
 
Unit 5 : STI and Opportunistic Infections Laboratory
  • Diagnosis of Syphilis
    • Samples referred from various hospitals of Delhi for serodiagnosis of syphilis
    • Subjected to Rapid Plasma Reagin (RPR) card test and Treponema pallidum Hemeagglutination (TPHA) test
  • Detection of pathogens causing diarrhea in HIV/ AIDS
    • Stools samples are referred from various hospitals of Delhi for detection of opportunistic diarrheal pathogens
    • Subjected to modified ZN staining for detection of Cryptosporidium parvum, Isospora belli and Cyclospora cayetanensis
  • Diagnosis of pneumonia due to Pneumocystis jeroveci
    • Respiratory samples (sputum, bronchoalveolar lavage) are subjected to Giemsa and Gomori’s Methanamine Silver staining for diagnosis of pneumonia due to P. jerovec
 
Unit 6 : Molecular Virology Laboratory
  • HIV viral load assay by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) performed on  samples as part of collaborative research project entitled “Comparative study on HIV/AIDS with anti retroviral and add on Homoeopathy drugs” with Central Council for Research in Homoeopathy (CCRH), Department of AYUSH, New Delhi.
 
Unit 7 : Integrated Counseling and Testing Centre (ICTC)
  • The client is counseled and tested for HIV, on his/her own free will or as advised by a treating doctor.
  • The main functions include:
    • Early detection of HIV infection.
    • Provision of basic information on modes of transmission and prevention of HIV/AIDS
    • Promoting behavioral change and reducing vulnerability
    • Link people with other HIV prevention, care and treatment services
    • Participation in External Quality Assessment Programme (EQAS) for HIV serology conducted by the linked SRL.
 
Unit 8 : Central Sample Collection Unit
  • Clinical samples are collected from the patients for all the divisions of NCDC and then distributed to the respective divisions
  • Reports are collected from all the divisions of NCDC and given to respective patients.
 
Unit 9 : Test Kits Distribution Unit
  • The cold room on the Ground floor of Lab Block acts as the Kit Distribution Unit for DSACS.
  • Kits of HIV, HBV and HCV are received and distributed by DSACS to various Centers of Delhi.
 
Significant Achievement
  • The centre was awarded the certificate of achievement for outstanding performance as a Training Institute by Delhi State AIDS Control Society (DSACS) on 1st November, 2008, on the Occasion of the 11th Foundation Day of DSACS.
  • The centre is giving 100% concordant results in various External Quality Assessment Schemes (EQAS) for past many years.
  • The National Reference Laboratory at this centre has been identified as one of the five NRLs to be entrusted with the task of HIV/HBV/HCV kit evaluation by National AIDS Control Organization.
  • The centre has been designated as one of the laboratory for testing of Dried Blood Spot (DBS) samples under HIV Sentinel Surveillance programme of National AIDS Control Organization.
 

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